Alpha-synuclein expression in substantia nigra and cortex in Parkinson's disease.
Identifieur interne : 004C01 ( Main/Exploration ); précédent : 004C00; suivant : 004C02Alpha-synuclein expression in substantia nigra and cortex in Parkinson's disease.
Auteurs : M. Neystat [États-Unis] ; T. Lynch ; S. Przedborski ; N. Kholodilov ; M. Rzhetskaya ; R E BurkeSource :
- Movement disorders : official journal of the Movement Disorder Society [ 0885-3185 ] ; 1999.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Cerebral Cortex (metabolism), DNA, Recombinant (genetics), Female, Gene Expression (genetics), Humans, Male, Middle Aged, Nerve Tissue Proteins (biosynthesis), Nerve Tissue Proteins (genetics), Parkinson Disease (genetics), Parkinson Disease (metabolism), Phosphoproteins (biosynthesis), Phosphoproteins (genetics), Point Mutation (genetics), Substantia Nigra (metabolism), Synucleins, alpha-Synuclein.
- MESH :
- chemical , biosynthesis : Nerve Tissue Proteins, Phosphoproteins.
- chemical , genetics : DNA, Recombinant, Nerve Tissue Proteins, Phosphoproteins.
- genetics : Gene Expression, Parkinson Disease, Point Mutation.
- metabolism : Cerebral Cortex, Parkinson Disease, Substantia Nigra.
- Adult, Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Synucleins, alpha-Synuclein.
Abstract
Mutations in the human alpha-synuclein gene have been identified in several families of European descent with early-onset Parkinson's disease (PD). We sequenced the complete alpha-synuclein cDNA from substantia nigra and cortex from nine patients with PD and eight control subjects. No mutations were found. We then analyzed alpha-synuclein mRNA levels using a ribonuclease protection assay. Two major protected bands of alpha-synuclein mRNA, possibly representing two splice variants of the gene, were observed. Alpha-synuclein mRNA was significantly diminished in the substantia nigra of patients with PD compared with control subjects but not in the cortex. Our findings suggest that decreased synuclein mRNA may be an early alteration in the SN in PD, and imply that decreased levels of the protein may play a role in the pathogenesis of sporadic cases of the disease.
PubMed: 10348463
Affiliations:
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Le document en format XML
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Lynch</name></author><author><name sortKey="Przedborski, S" sort="Przedborski, S" uniqKey="Przedborski S" first="S" last="Przedborski">S. Przedborski</name></author><author><name sortKey="Kholodilov, N" sort="Kholodilov, N" uniqKey="Kholodilov N" first="N" last="Kholodilov">N. Kholodilov</name></author><author><name sortKey="Rzhetskaya, M" sort="Rzhetskaya, M" uniqKey="Rzhetskaya M" first="M" last="Rzhetskaya">M. Rzhetskaya</name></author><author><name sortKey="Burke, R E" sort="Burke, R E" uniqKey="Burke R" first="R E" last="Burke">R E Burke</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10348463</idno><idno type="pmid">10348463</idno><idno type="wicri:Area/PubMed/Corpus">004210</idno><idno type="wicri:Area/PubMed/Curation">004210</idno><idno type="wicri:Area/PubMed/Checkpoint">004274</idno><idno type="wicri:Area/Ncbi/Merge">000042</idno><idno type="wicri:Area/Ncbi/Curation">000042</idno><idno type="wicri:Area/Ncbi/Checkpoint">000042</idno><idno type="wicri:doubleKey">0885-3185:1999:Neystat M:alpha:synuclein:expression</idno><idno type="wicri:Area/Main/Merge">007881</idno><idno type="wicri:Area/Main/Curation">004C01</idno><idno type="wicri:Area/Main/Exploration">004C01</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alpha-synuclein expression in substantia nigra and cortex in Parkinson's disease.</title><author><name sortKey="Neystat, M" sort="Neystat, M" uniqKey="Neystat M" first="M" last="Neystat">M. Neystat</name><affiliation wicri:level="4"><nlm:affiliation>Department of Neurology, College of Physicians & Surgeons, Columbia University, Columbia-Presbyterian Medical Center, New York, NY 10032, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurology, College of Physicians & Surgeons, Columbia University, Columbia-Presbyterian Medical Center, New York, NY 10032</wicri:regionArea><placeName><region type="state">État de New York</region><settlement type="city">New York</settlement></placeName><orgName type="university">Université Columbia</orgName></affiliation></author><author><name sortKey="Lynch, T" sort="Lynch, T" uniqKey="Lynch T" first="T" last="Lynch">T. Lynch</name></author><author><name sortKey="Przedborski, S" sort="Przedborski, S" uniqKey="Przedborski S" first="S" last="Przedborski">S. Przedborski</name></author><author><name sortKey="Kholodilov, N" sort="Kholodilov, N" uniqKey="Kholodilov N" first="N" last="Kholodilov">N. Kholodilov</name></author><author><name sortKey="Rzhetskaya, M" sort="Rzhetskaya, M" uniqKey="Rzhetskaya M" first="M" last="Rzhetskaya">M. Rzhetskaya</name></author><author><name sortKey="Burke, R E" sort="Burke, R E" uniqKey="Burke R" first="R E" last="Burke">R E Burke</name></author></analytic><series><title level="j">Movement disorders : official journal of the Movement Disorder Society</title><idno type="ISSN">0885-3185</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Cerebral Cortex (metabolism)</term><term>DNA, Recombinant (genetics)</term><term>Female</term><term>Gene Expression (genetics)</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Nerve Tissue Proteins (biosynthesis)</term><term>Nerve Tissue Proteins (genetics)</term><term>Parkinson Disease (genetics)</term><term>Parkinson Disease (metabolism)</term><term>Phosphoproteins (biosynthesis)</term><term>Phosphoproteins (genetics)</term><term>Point Mutation (genetics)</term><term>Substantia Nigra (metabolism)</term><term>Synucleins</term><term>alpha-Synuclein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nerve Tissue Proteins</term><term>Phosphoproteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term><term>Nerve Tissue Proteins</term><term>Phosphoproteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression</term><term>Parkinson Disease</term><term>Point Mutation</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cerebral Cortex</term><term>Parkinson Disease</term><term>Substantia Nigra</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Synucleins</term><term>alpha-Synuclein</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mutations in the human alpha-synuclein gene have been identified in several families of European descent with early-onset Parkinson's disease (PD). We sequenced the complete alpha-synuclein cDNA from substantia nigra and cortex from nine patients with PD and eight control subjects. No mutations were found. We then analyzed alpha-synuclein mRNA levels using a ribonuclease protection assay. Two major protected bands of alpha-synuclein mRNA, possibly representing two splice variants of the gene, were observed. Alpha-synuclein mRNA was significantly diminished in the substantia nigra of patients with PD compared with control subjects but not in the cortex. Our findings suggest that decreased synuclein mRNA may be an early alteration in the SN in PD, and imply that decreased levels of the protein may play a role in the pathogenesis of sporadic cases of the disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>New York</li></settlement><orgName><li>Université Columbia</li></orgName></list><tree><noCountry><name sortKey="Burke, R E" sort="Burke, R E" uniqKey="Burke R" first="R E" last="Burke">R E Burke</name><name sortKey="Kholodilov, N" sort="Kholodilov, N" uniqKey="Kholodilov N" first="N" last="Kholodilov">N. Kholodilov</name><name sortKey="Lynch, T" sort="Lynch, T" uniqKey="Lynch T" first="T" last="Lynch">T. Lynch</name><name sortKey="Przedborski, S" sort="Przedborski, S" uniqKey="Przedborski S" first="S" last="Przedborski">S. Przedborski</name><name sortKey="Rzhetskaya, M" sort="Rzhetskaya, M" uniqKey="Rzhetskaya M" first="M" last="Rzhetskaya">M. Rzhetskaya</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Neystat, M" sort="Neystat, M" uniqKey="Neystat M" first="M" last="Neystat">M. Neystat</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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